coli or Salmonella and One more 100 ml of peptone water inoculate with Staph. aureus or Ps. aeruginosa and transfer the membrane to 100 ml of sterile nutrient broth and soybean casein digest medium respectively.ICH: Q 4 B Annex 4A: To notice for evaluation and advice of pharmacopoeial texts to be used within the ICH regions on microbiological asses